Journal: Antioxidants

Article Title: The Protective Efficacy of Total Alkaloids from Nelumbinis Plumula on Irradiation-Induced Oxidative Stress Damage in Human Umbilical Vein Endothelial Cells

doi: 10.3390/antiox15010049

Figure Lengend Snippet: NPAE exerts antioxidative stress effects by enhancing cell viability, suppressing H 2 O 2 -induced ROS accumulation, and protecting against DNA damage. HUVECs received NPAE pretreatment (5–20 μg/mL) 2 h prior to 600 μM H 2 O 2 . Schematic representation of NPAE pretreatment experiments ( A ). Cell viability assay at 48 h post-treatment ( B , C ). ELISA analysis of 8-OHdG level at 4 h post-treatment ( D ). DAPI/γ-H2AX co-staining visualized DNA damage with fluorescence intensity analysis at 4 h post-treatment ( E ). DHE/Hoechst 33,342 co-staining visualized ROS accumulation with fluorescence intensity analysis at 24 h post-treatment ( F ). * p < 0.05, ** p < 0.01, *** p < 0.001 vs. IR group; ns indicated non-significant.

Article Snippet: Simultaneously, the content of 8-hydroxy-2′-deoxyguanosine (8-OHdG) in the cell culture supernatant was measured using a commercial enzyme-linked immunosorbent assay (ELISA) kit (Elabscience, Wuhan, China; Catalog No. E-EL-0028).

Techniques: Viability Assay, Enzyme-linked Immunosorbent Assay, Staining, Fluorescence

Journal: Antioxidants

Article Title: The Protective Efficacy of Total Alkaloids from Nelumbinis Plumula on Irradiation-Induced Oxidative Stress Damage in Human Umbilical Vein Endothelial Cells

doi: 10.3390/antiox15010049

Figure Lengend Snippet: NPAE protects endothelial cells against IR-induced damage by suppressing the pyroptosis pathway through downregulation of key executioner proteins. Schematic representation of NPAE pretreatment experiments ( A ). Caspase-1/PI dual staining quantifying early (Q2: caspase-1 +/PI −) and late (Q3: caspase-1 +/PI +) pyroptotic populations at 72 h post-irradiation ( B ). Western blot detection of pro-caspase-1, its activated fragment cleaved caspase-1 and GSDMD proteolytic processing quantification at 72 h post-irradiation ( C ). ELISA analysis of IL-18 secretion ( D ) and IL-1β secretion ( E ) 96 h post-irradiation. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. IR group; ns indicated non-significant; GAPDH served as loading control for immunoblotting.

Article Snippet: Simultaneously, the content of 8-hydroxy-2′-deoxyguanosine (8-OHdG) in the cell culture supernatant was measured using a commercial enzyme-linked immunosorbent assay (ELISA) kit (Elabscience, Wuhan, China; Catalog No. E-EL-0028).

Techniques: Staining, Irradiation, Western Blot, Enzyme-linked Immunosorbent Assay, Control

Journal: Pharmaceuticals

Article Title: Protection by Vitis vinifera L. Against Cisplatin-Induced Testicular Injury: Oxidative Stress, Inflammation, and Ferroptosis

doi: 10.3390/ph19010178

Figure Lengend Snippet: Effect of Vitis vinifera extract treatment on the protein levels of ( A ) nuclear factor erythroid2-related factor2 (NRF2), ( B ) heme Oxygenase-1 (HO-1), ( C ) glutathione peroxidase 4 (GPX4), ( D ) 8-Hydroxy-2′-deoxyguanosine (8-OHdG), ( E ) nuclear factor kappa B (NF-KB), ( F ) interleukin (IL)-1B, ( G ) tumor necrosis factor alpha (TNF)-α level, ( H ) ferritin, and ( I ) Cathepsin B levels against Cisplatin (Cisp)-induced testicular toxicity in a rat model. Tukey’s test was used to examine the data after a one-way ANOVA, and the mean ± SE (n = 5) was used to express the result that was obtained. The following symbols indicate statistical significance: ns = nonsignificant, * = p < 0.05, ** = p < 0.01, *** = p < 0.001, and **** = p < 0.0001.

Article Snippet: The rat 8_hydroxy_desoxyguanosine (8-OHdG) was quantitatively measured in the testicular homogenate using 8-OHdG ELISA KIT CUSABIO Co., Cat. No. (CSB_E10526r).

Techniques: